The game is set immediately after 'Splosion Man, when Big Science finally manages to capture its experiment, 'Splosion Man. As the scientists celebrate their accomplishment in a laboratory, one scientist accidentally spills champagne over some wiring, which sends a surge of electricity to a nearby platform where a yellow bow from another bottle had fallen. From the energy bow, Ms. 'Splosion Man is created, wearing the same yellow bow on her head.
 Game Play
Ms. 'Splosion Man is made of the same explosive matter as 'Splosion Man, which allows her to create explosions in the same matter in order to overcome various obstacles and traps.
New gameplay elements have been added to Ms. 'Splosion Man. Players can now ride along hanging rails to avoid hazards and use directional cannons to overcome great distances. Unlike the previous game, Ms. 'Splosion Man features various new environments taking place outside the laboratory.