Complete each achievement to get the allotted gamerscore. The numbers between the () is the points given for each achievement.
| Achievement | How to unlock |
|---|
| Technical Difficulties (10) | Attempt to access DNA Sequence 9 for the first time. |
| Battle Wounds (20) | Complete DNA Sequence 1. |
| Sanctuary! Sanctuary! (20) | Find a secure place to hide and re-enter the Animus. |
| Rome in Ruins (20) | Complete DNA Sequence 2. |
| Fixer-Upper (20) | Complete DNA Sequence 3. |
| Principessa in Another Castello (20) | Complete DNA Sequence 4. |
| Fundraiser (20) | Complete DNA Sequence 5. |
| Forget Paris (20) | Complete DNA Sequence 6. |
| Bloody Sunday (20) | Complete DNA Sequence 7. |
| Vittoria Agli Assassini (20) | Complete DNA Sequence 8. |
| Requiescat In Pace (20) | Complete DNA Sequence 9. |
| A Knife to the Heart (50) | Secure the Apple of Eden. |
| Perfect Recall (30) | Achieve 100% Synchronization with a Sequence. |
| Déjà vu (20) | Replay a Memory. |
| Undertaker 2.0 (20) | Discover the Shrine in the Catacombe di Roma. |
| Golden Boy (20) | Discover the Shrine in the Terme di Traiano. |
| Gladiator (20) | Discover the Shrine in Il Colosseo. |
| Plumber (20) | Discover the Shrine in the Cloaca Maxima. |
| One-Man Wrecking Crew (20) | Discover the Shrine in Palazzo Laterano. |
| Amen (20) | Discover the Shrine in the Basilica di San Pietro. |
| Bang! (20) | Destroy the Machine Gun. |
| Splash! (20) | Destroy the Naval Cannon. |
| Boom! (20) | Destroy the Bomber. |
| Kaboom! (20) | Destroy the Tank. |
| Home Improvement (20) | Upgrade 5 buildings in the Antico district. |
| Tower Offense (20) | Burn All Borgia Towers. |
| Show Off (20) | Complete 10 Guild Challenges. |
| .. .- -- .- .-.. .. ...- . (20) | Solve all of Subject 16’s Puzzles. |
| Perfectionist (20) | Earn 3 gold medals from the Animus Virtual Training Program |
| Brotherhood (20) | Recruit 3 Assassins. |
| Welcome to the Brotherhood (20) | Train a recruit up to the Rank of Assassin. |
| Capture the Flag (30) | Remove All Borgia Flags in Rome. |
| In Memoriam (20) | Collect All Feathers. |
| Dust to Dust (20) | Find 1 Artifact in the Present. |
| Serial Killer (20) | Perform a Speed Kill Streak of 10. |
| Spring Cleaning (10) | Kill a guard with a broom. |
| Your Wish is Granted (10) | Throw money in a well |
| Fly Like an Eagle (10) | Jump with the parachute off the top of Castel Sant' Angelo. |
| The Gloves Come Off (10) | Win the highest bet at the Fights. |
| Mailer Daemon (20) | Access your email in 2012 |
| Synchronization Established (10) | Complete an entire Session with at least one kill (Multiplayer Only). |
| Needle in a Haystack (5) | Kill your target while hidden in a hay bale (Multiplayer Only). |
| Strong Closer (20) | Take the lead 10 seconds before the end of the session and go on to win (Multiplayer Only). |
| Fast Learner (25) | Kill your target and escape your pursuer in less than 10 seconds (Multiplayer Only). |
| Job skills (20) | During Open Conflict, kill your target and escape (Multiplayer Only). |
| Download Complete (40) | Reach Level 50 (Multiplayer Only). |
| Role Model (20) | Get all the Co-op bonuses in 1 session (Multiplayer Only). |
| Overachiever (20) | Score 750 points or more on a single kill (Multiplayer Only). |
| Abstergo Employee of the Month (20) | Get every bonus at least once (Multiplayer Only). |
| Ahead of the Curve (20) | Perform a double or a triple escape (Multiplayer Only). |
| DLC Achievements | The Da Vinci Disappearance |
| Strong-Arm (10) | Throw a Long Weapon, Heavy Weapon, and Smoke bomb more than 10 m at a guard. |
| High Roller (20) | Win 10000 florins playing Hazard. |
| il Principe (100) | Receive 100% synch in ACB and full synch in all missions of the Da Vinci Disappearance. |
| Airstrike (20) | Kill 10 guards with a single arrow storm. |
| GPS (20) | Complete the Da Vinci Disappearance. |
| Clowning Around (30) | Beat up the 5 thirsty harlequins. |
| Special Delivery (20) | Double Assassinate from a parachute. |
| Grand Theft Dressage (20) | Steal 5 horses from their riders, while remaining on horseback. |
| Going Up (5) | Kill a guard with the bag that drops from a lift. |
| Easy Come, Easy Go (5) | Pay 500 florins to an orator, then pickpocket him afterward. |
