| Unlockable | How to unlock |
| Anko | Complete Story Mode on Difficulty 2 , Power 2 then buy from shop. |
| Chiyo | Complete Story Mode on Difficulty 2 , Power 2 then buy from shop. |
| Choji | Complete Story Mode on Difficulty 2 , Power 2 then buy from shop. |
| Deidara | Complete Story Mode on Difficulty 2 , Power 2 then buy from shop. |
| Hinata | Complete Story Mode on Difficulty 2 , Power 2 then buy from shop. |
| Hiruko | Complete Story Mode on Difficulty 2 , Power 2 then buy from shop. |
| Kiba | Complete Story Mode on Difficulty 2 , Power 2 then buy from shop. |
| Sasori | Complete Story Mode on Difficulty 2 , Power 2 then buy from shop. |
| Shikamaru | Complete Story Mode on Difficulty 2 , Power 2 then buy from shop. |
| Shino | Complete Story Mode on Difficulty 2 , Power 2 then buy from shop. |
| Baki | Finish Story Mode, then buy from shop. |
| Asuma | Finish Story Mode, then buy from shop. |
| Itachi | Finish Story Mode, then buy from shop. |
| Kisame | Finish Story Mode, then buy from shop. |
| Kurenai | Finish Story Mode, then buy from shop. |
| Temari | Finish Story Mode, then buy from shop. |
| ANBU Kakashi | Complete 40% of all the Missions including the S-Rank mission for Kakashi, and 2 other Missions for every one else, then redo Kakashi's S-Rank mission and complete it. Then buy from shop. |
| Towa | Have a save file from Naruto Clash of Ninja Revolution 2 then buy from the shop. If no save file, buy all the starting character options, exit shop, go back and buy. |
| Komachi | Same as Towa |
| Kagura | Same as Towa and Komachi |
| Bando | Buy everything in shop besides Anbu Kakashi and himself. Then have 70,000 ryo and go to shop. However, Bando only costs 35,000 ryo. |
| Kakuzu | Complete 30% of missions for Deidara, Sasori, Hiruko, Itachi, and Kisame. Then buy at shop |
| Hidan | Complete 30% of missions for Deidara, Sasori, Hiruko, Itachi, and Kisame. Then buy from shop. |
| Sasuke Uchiha | Buy every character aside from Kakazu and Hiden. Exit and re-enter shop to buy Sasuke. |